Enhancing the Production
of a Novel Exopolysaccharide by Bacillus
mucilaginosus CGMCC5766 Using Statistical Experiment
Design
Hui Li1, Jie Li1,
Wenfang Dou1, Jinsong Shi1* and
Zhenghong Xu1
1
Laboratory of
Pharmaceutical Engineering, School of Pharmaceutical
Science, Jiangnan University, 2 The
Key Laboratory of Industrial Biotechnology, Ministry of
Education, Jiangnan University, Wuxi 214122, PR China
*For correspondence:
Email:
shijs@163.com; Tel:
+86-510-85328177; Fax: +86-510-85328177
Received: 18 October 2012
Revised accepted: 25 July 2013
Tropical Journal of
Pharmaceutical Research, October 2013;
12(5):
711-718
http://dx.doi.org/10.4314/tjpr.v12i5.8
Abstract
Purpose: To improve the production of a
novel exopolysaccharide (EPS) by Bacillus mucilaginosus
CGMCC5766.
Methods: The culture medium for production
of EPS was optimized using statistical experiment
design. Sucrose, CaCO3 and K2HPO4
were found to be the key factors based on the results
obtained from Plackett�Burman experimental design. The
optimal concentration range of the three factors was
examined by the steepest ascent path, and then the
optimum concentration was further investigated through
response surface methodology. Validation experiments
were also carried out to establish the adequacy and
accuracy of the model obtained.
Results:
Under optimal conditions (sucrose 61.31 g/l, CaCO3
5.06 g/l and K2HPO4 0.17
g/l), EPS concentration achieved 22.04 g/l, which was
about 1.6-fold increase compared with that using the
original medium. EPS concentration in 5-l fermenter
reached 23.46 � 0.76 g/l.
Conclusion: The optimization medium is
considered fundamental and useful for the development of
B. mucilaginosus CGMCC5766 cultivation process for
efficient production of EPS on a large scale.
Keywords: Bacillus
mucilaginosus CGMCC5766, Exopolysaccharide, Medium,
Optimization, Statistical experiment design
