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Original Research Article
Ginger Essential Oil
Ameliorates Cisplatin-Induced Nephrotoxicity in Mice
Marcio J Dami�o1,
Gisele Giannocco2, Renata Grespan1,
Expedito L Silva3, Janaina S Duarte2,
Rui MB Maciel2, Alcil�ia N Yamada1,
Ciomar A Bersani-Amado1 and Roberto KN Cuman1*
1Department of Pharmacology
and Therapeutics, State University of Maring�, Maring�,
Paran�, 2Department of Endocrinology, Federal
University of S�o Paulo, CEP 04039002, S�o Paulo, 3Department
of Chemistry, State University of Maring�, Maring�,
Paran�, Brazil
*For correspondence:
Email:
rkncuman@uem.br; Tel:
55-44-30114867; Fax: 55-44-30114999.
Received: 25 June 2013 Revised accepted: 16 October
2013
Tropical Journal of
Pharmaceutical Research, December 2013;
12(6): 959-965
http://dx.doi.org/10.4314/tjpr.v12i6.14
Abstract
Purpose: To investigate the effect of ginger
essential oil (GEO) in an experimental model of
cisplatin-induced nephrotoxicity.
Methods: Male mice were divided into
treatment six groups (n = 7), namely: Groups I (saline),
II and III (cisplatin, 10 mg/kg, i.p.) euthanized in 3th
and 6th days, respectively, and IV, V and IV
(GEO, 100, 200 and 400 mg/kg/day, respectively, by
gavage 3, 4, 5 and 6 days after cisplatin injection).
Creatinine levels and protein/creatinine ratio were
determined in plasma and urine, respectively. Bone
morphogenic protein (BMP-7) and tumor necrosis factor (TNF-a)
levels of kidney tissues were determined while mRNA
expression levels were obtained using real-time
polymerase chain reaction.
Results: GEO treatment reduced significantly
creatinine levels to 0.53 � 0.02; 0.48 � 0.008
and 0.46 � 0.02 at 100, 200 and 400 mg/kg, respectively,
compared with control (0.70 � 0.01) [p<0.05] but
increased protein : creatinine ratio to 0.21 � 0.01,
0.22 � 0.01, 0.24 � 0.02 compared with control (0.06 �
0.008) [p<0.05]. Pro-inflammatory TNF-a mRNA expression
was decreased to 1.46 � 0.21, 1.39 � 0.19 and 1.36 �
0.09, at GEO doses of 100, 200 and 400 mg/kg,
respectively, while anti-fibrotic BMP-7 mRNA expression
increased to 2.05 � 0.26 and 2.44 � 0.42 at doses of 200
and 400 mg/kg, respectively, compared with control (0.59
� 0.39, p < 0.05).
Conclusion: GEO treatment attenuates cisplatin-induced
nephrotoxicity, in part, by modulating some inflammatory
cytokines.
Keywords: Zingiber officinale,
Ginger, Roscoe, Essential oil, Nephrotoxicity, Cisplatin. |