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Original Research Article
Assessment of
Antidermatophytic Activities of Urtica dioica L
against Microsporum canis in a Guinea Pig
Model
Ali Mikaeili1,
Isaac Karimi2 ⃰, Masoud
Modaresi3 and Zahra Bagherinasab3
1Department of Medical
Mycology, School of Medicine, Kermanshah University of
Medical Sciences, Kermanshah,, 2Division of
Biochemistry, Physiology and Pharmacology, Department of
Basic Veterinary Sciences, School of Veterinary
Medicine, Razi University, Kermanshah, 3Department
of Pharmacognosy and Pharmaceutical Biotechnology,
School of Pharmacy, Kermanshah University of Medical
Sciences, Kermanshah, Iran.
*For correspondence:
Email:
isaac_karimi2000@yahoo.com;
karimiisaac@razi.ac.ir; Tel:
+98 831 8322599; Fax: +98 831 8320041.
Received:18 October 2013 Revised accepted: 5 October
2013
Tropical Journal of
Pharmaceutical Research, December 2013;
12(6): 997-1002
http://dx.doi.org/10.4314/tjpr.v12i6.19
Abstract
Purpose: To investigate the antidermatophytic
effects of Urtica dioica L. against Microsporum canis in
vitro and in a guinea pig model.
Methods: The in vitro antifungal activity of
the extracts was measured by disc diffusion method and
assessment of minimum inhibitory concentration (MIC).
The dorsum of the guinea pigs was infected with M. canis
and topically treated as follows: negative control (NC)
received no treatment; positive control (PC) received
terbinafine 1 %; DMSO group (negative control) received
10 % DMSO (vehicle); while three other groups, viz,
SN10%, SN20% and SN30% received 10, 20 and 30 %
hydroalcohol extract dissolved in 10 % DMSO,
respectively.
Results: Both extracts did not show
considerable antifungal activity against M. canis
compared with terbinafine. The MICs of aqueous and
hydroalcoholic extracts were 30 and 20 mg.ml-1,
respectively. PC and SN30% groups showed normal hair
growth on day 15 while their skin looked healthy on day
30 post-inoculation. Lesion severity and clinical scores
declined in SN30% and PC groups in comparison to NC
group, on day 30 post-inoculation. Also, on day 30
post-inoculation, the clinical efficacy of the test
formulations increased to 11.76, 23.52, 76.47, 5.88, and
94.11 % for SN10%, SN20%, SN30%, DMSO10%, and PC groups,
respectively.
Conclusions: The hydroalcoholic extract of this
plant could be further developed, with a view to its
possibe use as an adjunct remedy for topical treatment
of tinea corporis.
Keywords: Urtica dioica L., Microsporum canis,
Kurdish Ethnomedicine, Antifungal, Hair growth, Lesion,
Dermatophytosis, Tinea corporis |
