Effect of Corynoline
Isolated from Corydalis bungeana Turcz on
Lipopolysaccharides-Induced Sepsis In vivo and
In vitro
Zhi-biao He1,
Ping Chen2, Zhen-yu Peng1 and Li-yan
Jin3*
1Department
of Emergency, 2Department of Pulmonary
Medicine, 3Department of Anesthesia, The
Second Xiangya Hospital of Central South University,
Changsha, Hunan 410011, PR China
*For correspondence:
Email:
lyanjmsu@126.com;
Tel/Fax:
+86 0731-85295970
Received: 11 September 2013
Revised accepted: 18 November 2013
Tropical
Journal of Pharmaceutical Research, January 2014; 13(1):
81-86
http://dx.doi.org/10.4314/tjpr.v13i1.12
Abstract
Purpose: To investigate the
protective effect of corynoline isolated from Corydalis
bungeana Turcz on lipopolysaccharides (LPS)-induced
sepsis, and determine the possible mechanism of
anti-sepsis effect of the isolated corynoline.
Methods: Corynoline was extracted by
column chromatography. LPS (100 ng/mL) was used to
induce the release of TNF-α and IL-6 in RAW 264.7 cells,
and the isolated corynoline was added. ELISA method was
used to determine the levels of TNF-α and IL- 6.
Furthermore, sepsis in mice was established by injection
of LPS (2 mg/kg, i.v.), and the levels of TNF-α and IL-6
in plasma were determined by ELISA method. For survival
rate test, LPS (15 mg/kg, i.v.) and heat-killed E. coli
(1.0 ×1011 CFU/kg, i.v.) were used to
establish sepsis in mice model, and the mice were
observed in 7 days.
Results:
The results indicate that
corynoline significantly elevated the survival rate of
septic mice induced by LPS and heat-killed E. coli, in a
dose-dependent manner (p < 0.05). Corynoline decreased
the release of TNF-α and IL-6 induced by LPS, in a
dose-dependent manner (p < 0.05).
Conclusion:
Treatment with corynoline
significantly inhibits the mortality of LPS-induced
septic mice, and the mechanism of action is probably
related to the decrease of TNF-α and IL-6 release. Thus
corynoline has the potential to be developed as an
effective and safe drug for treating sepsis.
Keywords: Corynoline,
Corydalis bungeana, Sepsis, Lipopolysaccharides, TNF-α,
IL-6
