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Original Research Article
Buffer-Free High
Performance Liquid Chromatography Method for the
Determination of Theophylline in Pharmaceutical Dosage
Forms
Mahibub M Kanakal1-3*,
Aman Shah Abdul Majid 3, Munavvar Zubaid A
Sattar2, Noor Shahida Ajmi4 and
Amin Malik Shah Abdul Majid2
1
Department of Pharmaceutics,
Faculty of Pharmacy, UiTM-PP, Bertam Campus, Kepala
Batas,13200 P.Pinang, 2School of
Pharmaceutical Sciences, Universiti Sains Malaysia,
11400 P.Pinang, 3Cluster of Integrative
Medicine, Advanced Medical and Dental Institute,
Universiti Sains Malaysia, 4Department of
Pharmacy, Allianze University College of Medical
Sciences (AUCMS), 13200, Kepala Batas,13200 P.Pinang,
Malaysia.
*For correspondence: Email:
mehboobct@gmail.com; Tel:
00-60-194516823; Fax: 006-04-5623488
Received: 15 January 2013
Revised accepted: 30
November 2013
Tropical
Journal of Pharmaceutical Research, January 2014; 13(1):
149-153
http://dx.doi.org/10.4314/tjpr.v13i1.21
Abstract
Purpose:
To develop and validate a
simple, economical and reproducible high performance
liquid chromatographic (HPLC) method for the
determination of theophylline in pharmaceutical dosage
forms.
Method:
Caffeine was used as the
internal standard and reversed phase C-18 column was
used to elute the drug and standard. The mobile phase
was prepared by mixing water:acetonitrile:methanol at
the ratio of 90:03:07 and the pH set at 4.6. Flow rate
and ultraviolet (UV) detector were set at 1.0 mLmin-1
and 271 nm, respectively. The method was validated for
linearity, recovery, accuracy, precision, specificity,
and also for inter-day stability under laboratory
conditions.
Results:
Retention time was 5.5 min.
The limits of detection and quantification were 12.5
ngmL-1 and 100 ngmL-1,
respectively. Recovery accuracy
(%) for different concentrations ranged from 100.05 to
102.43; regression coefficient (R2)
of 0.994; precision RSD < 2.0, and negligible
interference from common excipients.
Conclusion: The method is simple,
rapid, highly specific and suitable for the
determination of theophylline. Absence of buffer and use
of small quantity of organic solvents increase the life
span of the column and reduce the cost of routine
analysis of theophylline in industry.
Keywords :
Buffer-free,
High performance liquid chromatography, Theophylline,
Dosage form. |
