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Original Research Article
Salvianolic Acid B Ameliorates Motor Dysfuntion in
Spinal Cord Injury Rats
Chong Xun, Shouyu Wang, Guang Chen, Yang Hu, Jiaqi Xie
and Decheng Lv*
Department of Surgery,
The First Affiliated Hospital of Dalian Medical
University, Dalian, 116000, PR China.
*For correspondence: Email: lvdecheng777@hotmail.com;
Tel: +86 0411-86724517,
Received: 2 February 2013 Revised accepted: 23 November
2013
Tropical
Journal of Pharmaceutical Research, January 2014; 13(1):
53-59
http://dx.doi.org/10.4314/tjpr.v13i1.8
Abstract
Purpose: To evaluate the effect of
salvianolic acid B (Sal B) treatment on the
motor function of spinal cord injury (SCI)
rat.
Methods: SCI rats were modelled by
contusion, and then received 10 mg/kg Sal B,
or methylprednisolone, or phosphate-buffered
saline (PBS) intraperitoneally daily for 4
weeks, two hours after the trauma occurred.
During the treatment, footprint analysis
(FA), inclined plane test (IPT),
Basso-Beattie-Bresnahan (BBB) rating and
Schnell Swim Test (SST) were used for
estimating the recovery of motor function.
At the same time, tissue edema was measured
by wet-dry weighting, and the secretion of
cytokines were indirectly quantitated by
real time polymerase chain reaction (qPCR).
Results: Primarily, Sal B group rats
scored higher by FA, IPT and BBB rating.
Further statistical analysis of
comprehensive SST data from Student-t test
indicates that Sal B can significantly
ameliorate motor dysfunction after a 4-week
treatment (p < 0.05) as well. Furthermore,
Sal B decreased water content of the edema
by 16.5 % during the first week, and sharply
downregulated the transcription of
interleukin 6 (IL-6) and tumor necrosis
factor (TNF-α) 28- and 16-fold,
respectively.
Conclusion: The beneficial effect of
motor function recovery was observed in SCI
rats following intraperitoneal
administration of Sal B.
Keywords: Salvianolic acid B, Spinal cord
injury, Motor dysfunction, Cytokines
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