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Original Research Article
Antidiarrhea and
Antioxidant Activities of Honokiol Extract from
Magnoliae officinalis cortex in Mice
Xuefeng Han1,
Yuelan Pang2,3, Shimin Liu4,
Zhiliang Tan1, Shaoxun Tang1,
Chuanshe Zhou1, Min Wang1 and
Wenjun Xiao2*
1Key Laboratory of
Agro-ecological Processes in Subtropical Region,
Institute of Subtropical Agriculture, The Chinese
Academy of Sciences, Changsha, Hunan 410125, 2National
Research Center of Engineering & Technology for
Utilization of Botanical Functional Ingredients, Hunan
Agricultural University, Changsha, Hunan 410128, 3Tea
Science and Research Institute of Guilin. Guangxi,
Guilin, Guangxi 541004, China, 4School of
Animal Biology, University of Western Australia,
Crawley, WA 6009 Australia
*For correspondence:
Email:
xiaowenjungong@163.com; Tel:
86-731-84673760; Fax: 86-731-84673760
Received: 14 May 2014
Revised accepted: 12
September 2014
Tropical
Journal of Pharmaceutical Research, October 2014;
13(10): 1643-1651
http://dx.doi.org/10.4314/tjpr.v13i10.11
Abstract
Purpose: To evaluate the
antidiarrhea and antioxidant properties of honokiol
extracted from Magnoliae officinalis cortex (bark of
Magnolia officinalis), an important medical material in
traditional Chinese medicine, for treating diseases such
as diarrhea and thrombotic stroke.
Methods: The antidiarrhea activity
of honokiol was investigated using castor oil-induced
diarrhea as well as neostigmine-induced increase in
small intestine transit in mice. In castor oil-induced
diarrhoea test, mice received honokiol (25, 50 and
100 mg/kg BW) orally once daily for 1 day and the mice’
droppings were observed. In small intestine transit
test, mice received honokiol (25, 50 and 100 mg/kg BW)
orally once daily for 4 days and the percentage distance
travelled by charcoal meal was noted to determine. For
the determination of anti-oxidant activity, with 50
mg/kg vitamin E as positive control, the mice were
administered with 25, 50 or 100 mg/kg honokiol orally
and daily for 14 days. The activity and gene expression
of antioxidative enzymes as well as antioxidant status
were monitored to assess the antioxidant potential of
honokiol.
Results: All doses of honokiol
showed (p < 0.001) significant inhibitory activity
against castor oil-induced diarrhea when compared with
model control (diarrhea Index, 1.10 vs. 1.39)-. Honokiol
at all doses also reduced neostigmine-stimulated small
intestinal transit by approximately 16 % in comparison
with -neostigmine control group-(59.0 % vs. 70.1%).
Compared with control (no honokiol), the activities of
catalase (CAT), glutathione peroxidase (GSH-Px) and
total superoxide dismutase (T-SOD) in the plasma (CAT,
7.31 vs. 13.21 U/mL; GSH-Px, 439.6 vs. 608.9 U/m; T-SOD,
82.2 vs. 109.8 U/mL) and liver (CAT, 7.73 vs. 14.39
U/mg; GSH-Px, 167.6 vs. 202.7 U/mg; T-SOD, 44.3 vs. 53.9
U/mg) were significantly enhanced by honokiol (p <
0.01). CAT and GSH-Px gene expressions were also
significantly enhanced by honokiol (p < 0.05), compared
with control (no honokiol) (CAT, 0.32 vs. 0.39; GSH-Px,
4.49 vs. 5.80). Additionally, total antioxidant capacity
was increased by 60 % with 100 mg/kg honokiol.
Conclusion: The results provide some
justification for the use of Magnoliae officinalis
cortex as an antidiarrheal remedy in Chinese traditional
medicine. The fact that honokiol also enhanced both the
non-enzymatic and enzymatic antioxidant defense systems,
suggests its potential as a natural antioxidant.
Keywords: Magnoliae officinalis
cortex, Honokiol, Antidiarrheal, Small intestinal
transit, Antioxidant, Gene expression |
