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Original Research Article
Vitamin D and IL28B
Genotyping as Predictors for Antiviral Therapy: A
Retrospective Study in Egyptian HCV Genotype 4a
Nadia Abdelaaty Abdelkader1,
Soha Saoud Abdelmoniem2, Dina Sabry3*,
Amin Mohamad Abdelbaky4, Maram M Mahdy5,
Eman Zaky5 and Wessam Elsayed Saad6
1Tropical Medicine Department,
Ain Shams University, Cairo, 2Tropical
Medicine & Gastroenterology Department, Assiut
University, Assiut, 3Medical Biochemistry and
Molecular Biology Department, Cairo University, 4Tropical
Medicine Department, National Hepatology and Tropical
Medicine Research Institute (NHTMRI), 5Internal Medicine
Department, Ain Shams University, 6Clinical
Pathology Deparment, Ain Shams University, Cairo, Egypt
*For correspondence:
Email:
dinasabry@kasralainy.edu.eg,
dinnasabry69@yahoo.com
Received: 18 July 2014
Revised accepted: 12
September 2014
Tropical
Journal of Pharmaceutical Research, October 2014;
13(10): 1 725-1732
http://dx.doi.org/10.4314/tjpr.v13i10.23
Abstract
Purpose: To evaluate the role of
pre-treatment vitamin D serum level and interleukin28B
(IL28B) (rs 12979860) polymorphism in chronic hepatitis
C (CHC) genotype 4a patients treated with pegylated
interferon α2-A and ribavirin (peg IFN+RBV) as
predictors of response.
Methods: A retrospective study of
clinical and pathological data and stored blood samples
of 150 naïve chronic hepatitis C (CHC) genotype 4a
patients, treated with pegylated interferon and
ribavirin for 48 weeks. Follow-up to detect sustained
virological response (SVR) was carried out. Based on SVR,
two groups were studied; group 1 consisted of 75
responder patients to pegylated IFN + RBV therapy while
group 2 comprised of 75 non-responder patients to
standard hepatitis C virus (HCV) therapy. Vitamin D
serum levels were assessed using Enzyme Linked
Immunoassay (ELISA), quantitative reverse transcriptase-
polymerase chain reaction (qRT-PCR for HCV RNA ), and
IL28B gene polymorphism by Restriction Fragment Length
Polymorphism Polymerase Cchain Reaction (RFLP-PCR).
Results: Pretreatment vitamin D
level was significantly higher in group 1 than in group
2 (p < 0.001). The sensitivity and specificity of
vitamin D level for prediction of SVR at a cutoff value
of 29.75 ng/ml were 100 and 96 %, respectively, with
area under the curve (AUC) of 0.995 (p < 0.001). A
significant difference was detected between baseline
vitamin D level for early versus advanced fibrosis stage
(p = 0.01) in group 1.
Conclusion: Pretreatment vitamin D
serum level (at a cutoff value of 29.75 ng/ml), IL28B
gene polymorphism and quantitative HCV RNA are
independent trait predictors of SVR.
Keywords: Vitamin D, Interleukin 28B,
Chronic hepatitis C, Sustained virological response (SVR),
Antiviral, Genotyping |
