Tropical Journal of Pharmaceutical Research

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Original Research Article

Isolation and Purification of Sesquiterpene Lactones from Ixeris sonchifolia (Bunge) Hance by High-Speed Counter-Current Chromatography and Semi-Preparative High Performance Liquid Chromatography

Wei Cai¹, Jia Yu Zhang², Guang Lei Li¹, Guo Ping Chang¹, Ya Lei Wei¹, Hong Gui Zhang¹* and Jian Qiu Lu²*

¹School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, ²Center of Scientific Experiment, Beijing University of Chinese Medicine, Beijing 100029, China

*For correspondence: Email: bzy714@163.com, lujq@vip.sina.com; Tel: +86 10 84738642; Fax: +86 10 84738642

Received: 9 July 2014 Revised accepted: 5 November 2014

Tropical Journal of Pharmaceutical Research, December 2014; 13(12): 2065-2069

http://dx.doi.org/10.4314/tjpr.v13i12.17

Abstract

Purpose: To isolate and purify sesquiterpene lactones from Ixeris sonchifolia (Bunge) Hance by high-speed counter-current chromatography (HSCCC).

Methods: I. sonchifolia was extracted with water and then loaded on a glass column (10 × 1500 cm containing 3000g D101 macroporous resin) where various concentrations of aqueous ethanol (0, 10, 30, 50, and 95 %) were used to elute the column successively. The 50 % ethanol fraction was purified by HSCCC using a solvent system comprised of ethyl acetate: n-butanol: methanol: water (4: 6: 1: 20, v/v), and semi-preparative high performance liquid chromatography (HPLC). The chemical structures of the components obtained were further confirmed by high-resolution mass spectroscopy (MS) and nuclear magnetic resonance spectroscopy (NMR).

Results: Three compounds, including ixerin Z1 (0.7 mg), ixerin Z (11.4 mg), and 11, 13α-dihydroixerin Z (8.2 mg), with purity of 96.2, 98.2, and 98.4 %, respectively, were obtained from 200 mg each of the 50 % ethanol fraction.

Conclusion: HSCCC is a rapid and effective method for isolating and purifying sesquiterpene lactones from I. sonchifolia.

Keywords: Sesquiterpene lactones, High-speed counter-current chromatography, Ixeris sonchifolia, Ixerin, 13α-Dihydroixerin