Polysaccharides from
Portulaca oleracea L Improve Exercise Endurance and
Decrease Oxidative Stress in Forced Swimming Mice
Chen Xiang1*,
Lan Zhang2, Zheng Xiaowei3 and Lou
Xiaojuan4
1Physical Education College,
Wenzhou University, Wenzhou 325035, 2Department
of Physical Education, Zhejiang Yuexiu University of
Foreign Languages, Shaoxing, 312000, 3Department
of Physical Education and Military Training, Zhejiang
University of Technology, Hangzhou, 310014, 4Physical
Education College, Donghua University, Shanghai 201620,
PR China.
*For correspondence:
Email:
tyxycx@sina.cn,
tyxycx@gmail.com; Tel: +86
13868551014; Fax: +86 0577 86680835
Received: 26 December 2012
Revised accepted: 5 January
2014
Tropical Journal of
Pharmaceutical Research, January 2014;
13(2):
229-234
http://dx.doi.org/10.4314/tjpr.v13i2.10
Abstract
Purpose:
To explore the effects of
polysaccharides from Portulaca oleracea L. (POP) on
exercise endurance and oxidative stress in
forced-swimming mice.
Methods:
Forty-eight mice were
divided into four groups of twelve animals each. All
treatments were administered orally and daily for 28
days. Group A received isotonic saline solution (50
ml/kg bodyweight) as control group; B, C and D groups
received 100, 200 and 400 mg/kg body wt. of
POP as treatment groups,
respectively. After the final treatment with
POP, the mice
were subjected to swimming to exhaustion and the
exhaustive swimming time, blood lactic acid (BLA), blood
glucose, malondialdehyde (MDA), superoxide dismutase
(SOD), glutathione peroxidase (GPx) and catalase (CAT)
were measured.
Results: The exhaustive swimming
time of the POP-treated
groups (967.3 ± 79.2, 1234.8 ± 97.6 and 1314.3 ± 107.3
s) was significantly longer than that of the control
group (513.6 ± 41.2 s) (p < 0.05). After the exhaustive
swimming exercise, BLA levels of the
POP-treated
groups (8.63 ± 0.91, 8.04 ± 0.86 and 7.51 ± 0.78 mmol/L)
were significantly lower than that of the control group
(11.39 ± 1.17 mmol/L) (p < 0.05). MDA levels of the
POP-treated
groups (2.69 ±
0.21,
2.41 ±
0.17 and
2.37 ±
0.23 U/mg.pro)
were significantly lower than that of the control group
(3.21 ±
0.29 U/mg.pro) (p
< 0.05). On the other hand, blood glucose levels of the
POP-treated
groups (5.47 ± 0.48, 5.74 ± 0.57 and 6.04 ± 0.51 mmol/L)
were significantly higher than that of the control group
(4.89 ± 0.32 mmol/L)
(p < 0.05).
SOD levels of the
POP-treated
groups (124.36 ± 14.87, 136.39±13.48 and 145.87 ± 17.39 U/mg.pro) were significantly higher than
that of the control group (108.41 ± 11.63
U/mg.pro)
(p < 0.05).
GPx levels of the
POP-treated
groups (68.24 ± 4.68, 71.33 ± 5.29 and 72.64 ± 5.99
U/mg.pro) were
significantly higher than that of the control group
(53.17 ± 5.24 U/mg.pro)
(p < 0.05). CAT levels of
the POP-treated
groups (23.57 ± 1.71, 24.28 ± 2.14 and 26.72 ± 2.21
U/mg.pro) were
significantly higher than that of the control group
(19.48 ± 2.03 U/mg.pr)
(p < 0.05).
Conclusion: This study provides
compelling evidence that POP can improve exercise
endurance and decrease oxidative stress in forced
swimming mice.
Keywords:
Polysaccharides, Portulaca oleracea L., Oxidative
stress, Swimming, Exercise endurance
