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Original Research Article
Central Nervous System
Depressant, Analgesic and Antidiarrheal Effects of the
Seed Extracts of Dimocarpus longan Lour in Rats
Farhana Alam Ripa1*,
Mahmud Tareq Ibn Morshed1, Afsana-Al-Sharmin2,
Shahed Bulbul Papon2, Md Rafiqul Islam2
and ZaraSheikh1
1Department
of Pharmacy, BRAC University, Mohakhali, 2Department
of Pharmacy, Southeast University, Banani, Dhaka-1213,
Bangladesh
.
*For correspondence:
Email:
ripa.seu@gmail.com; Tel:
+88-02-8912144, +88-01726216153
Received: 8 December 2012
Revised accepted: 14
December 2013
Tropical Journal of
Pharmaceutical Research, January 2014;
13(2):
235-242
http://dx.doi.org/10.4314/tjpr.v13i2.11
Abstract
Purpose: To assess the central
nervous system (CNS) depressant, analgesic and
antidiarrheal activities of the dried seed crude
extracts of Dimocarpus longan Lour in rodents.
Methods: Selected pharmacological
effects of the ethanol (ENLS), petroleum ether (PELS),
chloroform (CHLS) and ethyl acetate (EALS) extracts of
D. longan fruit seeds were investigated. CNS depressant
activity was evaluated by open field and hole cross
tests; analgesic activity by acetic acid-induced
writhing test and formalin-induced licking test; and
anti-diarrheal activity was assessed in castor oil and
magnesium-induced diarrhea rat model. The extracts were
given orally in a rat model at doses of 200 and 300
mg/kg body weight. Normal saline served as control in
all experiment. In CNS depressant test, diazepam (1
mg/kg) was used as reference drug while indomethacin (10
mg/kg) and loperamide (2 mg/kg) were used as standard
drugs in analgesic and antidiarrheal tests,
respectively.
Results: In hole cross method, EALS showed the most
effective depressant effect, viz, 1.170.17 for 200 mg/kg dose and 0.830.31 number of movements for
300 mg/kg dose after 120 min (p < 0.01), whereas in the
open field test, all the extracts exhibited significant
(p < 0.01) depressant effect in relation to positive
control, diazepam. In acetic acid-induced pain test,
PELS gave the lowest number of writhing (2.830.307) and the highest inhibition
(88.45 %, 300 mg/kg dose) which was statistically
significant. All the extracts also significantly (p <
0.01) suppressed licking activity in both phases of the
formalin-induced licking test, in contrast to
indomethacin. In the antidiarrheal tests, diarrheal
suppression was highest at 300 mg/kg dose for all the
extracts, compared with loperamide in both castor oil
and magnesium sulphate induced diarrhea model.
Conclusion:
The extracts of
Dimocarpus longan tested demonstrated significant CNS
depressant, analgesic and antidiarrheal activities in a
rodent model.
Keywords:
Dimocarpus longan Lour, CNS
depressant, Analgesic, Anti-diarrheal. |
