Tyrosinase Inhibitory Activity and Thermostability of
the Flavonoid Complex from Sophora japonica L (Fabaceae)
Jeng-Shiow Lai1, 2,
Chih-Chien Lin1*
and Tsung-Ming Chiang3
1Department
of Cosmetic Science, Providence University, No. 200,
Sec. 7, Taiwan Boulevard, Shalu, Taichung City, 43301,
2Department of Beauty Science, Chienkuo
Technology University, No. 1 Chiehshou North Road,
Changhua City 500, 3Panion &
BF Biotech Inc., 16 F, No. 3, Yuan. Chiu St., Nan Gang
Dist., Taipei 115, Taiwan, ROC.
*For correspondence:
Email:
chchlin@pu.edu.tw; Tel:
+886-4-26328001 ext 15409; Fax: +886-4-26311167.
Received: 8 December 2012
Revised accepted: 31
December 2013
Tropical Journal of
Pharmaceutical Research, January 2014;
13(2):
243-247
http://dx.doi.org/10.4314/tjpr.v13i2.12
Abstract
Purpose:
To
investigate the tyrosinase inhibitory activity and
thermostability of weak acid-treated Sophora japonica L.
flavonoid complex (SJ-FC) in different solutions.
Methods: The
flavonoid complex of S. japonica was isolated and
treated with weak acid to generate SJ-FC. The anti-tyrosinase
activities of SJ-FC and well-known tyrosinase inhibitors
were compared by mushroom tyrosinase activity assay. The
thermostabilities of SJ-FC and other inhibitors in
different solution environments for long-term storage
were also investigated.
Results: The
results indicate that SJ-FC has potent tyrosinase
inhibitory activity, and at a concentration of 0.1 %, SJ-DC
has a tyrosinase inhibitory activity equal to that of 1
% ascorbic acid or hydroquinone. In addition, SJ-FC in
both propylene glycol (PG) and glycerol solutions
exhibited obvious tyrosinase inhibitory activity.
Ascorbic acid and arbutin, two other tyrosinase
inhibitors, exhibit < 60 % of their initial activity in
both PG and H2O solutions after 6 months of
storage. However, SJ-FC stored in PG and H2O
solutions retained almost 100 % of its activity over a
6-month period.
Conclusion: SJ-FC is
an effective and stable anti-tyrosinase agent and may be
used as a function agent in medicines, foods and
cosmetics.
Keywords:
Flavonoid, Sophora japonica
L., Tyrosinase inhibitor, Thermostability
