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Original Research Article
Gas
Chromatography-Mass Spectrometric Analysis of Essential
Oil of Aerial Parts of Glycosmis parviflora
(Sims) Little (Rutaceae)
Zhi Long
Liu1*, Kai Yang1, Peng Hua Bai1, Ligang Zhou2, Shao Liang Liu3,
and Qi Zhi Liu1
1Department of Entomology,
2Department of Plant
Pathology, China Agricultural University, 2 Yuanmingyuan
West Road, Haidian District, Beijing 100193,China
3Department of Biology,
Faculty of Preclinical Medicine, GuangXi Traditional
Chinese Medical University, Nanning 530001, China.
*For correspondence:
Email:
zhilongliu@cau.edu.cn; Tel.:
+86-10-62732800; Fax: +86-10-62732800.
Received: 17 January 2013
Revised accepted: 6 January
2014
Tropical Journal of
Pharmaceutical Research, January 2014;
13(2):
275-280
http://dx.doi.org/10.4314/tjpr.v13i2.17
Abstract
Purpose: To investigate the chemical
composition and toxicity of the essential oil of
Glycosmis parviflora (Sims) Little aerial parts against
root-knot nematode and two grain storage insects (maize
weevil and red flour beetle).
Methods: Steam distillation of G.
parviflora was carried out using Clavenger apparatus in
order to extract its volatile oil. Gas
chromatography/mass spectrometric (GC/MS) analysis
(HP-5MS column) of the essential oil was performed and
the toxicity of the oil determined by contact test.
Results:
A total of 37 components
comprising 98.7 % of the essential oil were identified,
of which (Z)-caryophyllene (20.6 %), methyl isoeugenol
(11.1 %), (Z)-β-ocimene (8.9 %), α-cubebene (6.4 %),
nerolidol (5.4 %), aromandendrene (4.9 %) and γ-pyronene
(4.7 %) were found to be the major components. The
essential oil possessed strong nematicidal activity
against M. incognita with an LC50 value of
92.84 μg/ml. The essential oil of G. parviflora
exhibited strong contact toxicity against S. zeamais and
T. castaneum adults with LD50 values of 41.7
and 22.6 μg/adult, respectively.
Conclusion: The study indicates that
the essential oil of G. parviflora aerial parts has a
potential for development into a natural insecticide/nematicide
for control of nematodes and grain storage insects.
Keywords:
Glycosmis parviflora,
Essential oil, Meloidogyne incognita, Sitophilus zeamais,
Tribolium castaneum, Contact toxicity |
