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Original Research Article
Protective Effects of
Aqueous Extract of Sempervivum tectorum L (Crassulaceae)
on Aluminium-Induced Oxidative Stress in Rat Blood
Muselin Florin1,
Trif Alexandra1*, Stana Loredana Gabriela2,
Cristina Teodor Romeo3, Grăvilă Corina4,
Măcinic Ioan1 and Dumitrescu Eugenia3
1USAMVB Faculty of Veterinary
Medicine, Department of Toxicology, Vegetal Biology and
Medicinal Plants, 2University of Medicine and
Pharmacy “Victor Babeș”, 3USAMVB Faculty of
Veterinary Medicine, Department of Pharmacology, 4USAMVB
Faculty of Zootehny and Biotechnology, Department of
Biochemistry, Timișoara, România
*For correspondence:
Email:
al_trif@yahoo.com Tel:
+40256277076,
Received: 1 February 2013
Revised accepted: 8 January
2014
Tropical Journal of
Pharmaceutical Research, January 2014;
13(2):
179-184
http://dx.doi.org/10.4314/tjpr.v13i2.2
Abstract
Purpose: To examine the effect of
Sempervivum tectorum aqueous extract , as infusion, on
some biomarkers of oxidative stress induced by aluminium
exposure in Wistar albino rat.
Methods: The rats were randomly
divided into five groups and treated daily for 3 months
as follows: control received tap water; the 2nd
group was given 1 mg/L aluminium as aluminium sulphate
(AS) in drinking water daily; group 3, a combination of
1 mg/L AS and Sempervivum tectorum extract daily; the
fourth group, Sempervivum tectorum extract daily; and
fifth group 1 mg/L AS for three months and thereafter
Sempervivum tectorum extract for one month.
Results: Aluminium (Al) exposure
increased, compared to control group, the level of blood
Al (293.64 %, p < 0.0001), serum superoxide dismutase
(SOD, 14.84 %, p > 0.05), glutathione peroxidase (G-Px,
31.25 %, p < 0.001) and thiobarbituric reactive
substances (TBARS, 21.37 %, p < 0.001) but significantly
decreased catalase (CAT, 49.59 %, p < 0.001),
glutathione (GSH, 13.49 %, p < 0.001) and glutathione
reductase (GSH-r, 23.08 %, p < 0.0001) levels. These
results indicate that Al-induced oxidative stress in the
rats. Following administration of Sempervivum tectorum
extract, the levels of the oxidative stress biomarkers
were restored to normal, indicating good antioxidant
properties of the extract. Al blood level was also
significantly reduced by 33.45 % (p < 0.001) in rats
exposed to the extract. Rat body weight was not
significantly (p > 0.05) affected by the extract.
Conclusion: S. tectorum extract
possesses antioxidant activity against free radicals
produced in blood as a result of aluminium exposure.
Keywords:
Aluminium exposure,
Sempervivum tectorum, Biomarkers, Free radicals,
Oxidative stress. |
