Pectolinarigenin - A Flavonoid Compound from
Cirsium Japonicum
with Potential Anti-proliferation Activity in
MCF-7 Breast Cancer Cell
Mingqian Lu1,2,3, Qingzhi Kong1,4*,
Xinhua Xu2,3, Hongda Lu4, Zhongxin
Lu4, Wei Yu2,3, Bin Zuo2,3,
Jin Su2,3 and Rong Guo2,3
1Hubei
University of Chinese Medicine, Wuhan
430061, 2Institute
of Oncology, China Three Gorges University,
3Oncology
Department, Yichang Central Hospital,Yichang 443000,
4Wuhan
Central Hospital & Wuhan Institute of Oncology, Wuhan
430061, China.
*For correspondence:
Email:
lumqyc@sina.cn; Tel:
+86-0717-6486745; Fax: +86-0717-6486745
Received: 17 January 2013
Revised accepted: 6 January
2014
Tropical Journal of
Pharmaceutical Research, January 2014;
13(2):
(ESI-MS)
analyses. The anti-proliferation activity of
pectolinarigenin was also evaluated by
methylthiazolyldiphenyl-tetrazolium
bromide (MTT) and
colony formation assays in MCF-7 breast cancer cell.
Results:
Pectolinarigenin
showed
potent anti-proliferation activity by inducing apoptosis
and downregulation of Bcl2 expression in MCF-7 breast
cancer cell. Protein expression of Bcl2 was almost
completely inhibited upon the treatment with 25 mM
pectolinarigenin.
Conclusion: A rapid
method to isolate high purity pectolinarigenin from C.
japonicum and as well as evaluate its anti-proliferative
potential and induction of apoptosis in MCF-7 breast
cancer cells is presented for the first time. Induction
of apoptosis of MCF-7 is via downregulation of Bcl2
expression.
Keywords:
Cirsium japonicum,
Anti-proliferation activity, Pectolinarigenin, Breast
cancer, Apoptosis.
