Tropical Journal of Pharmaceutical Research

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Original Research Article

Development and Application of Kinetic Spectrophotometric Method for the Determination of Metronidazole

Vojkan M Miljkovic^1*, Danijela A Kostic²*, Zoran Z Bojanic¹, Biljana M.Kalicanin¹, Gordana M Kocic¹

¹Faculty of Medicine, ²Faculty of Sciences and Mathematics, The University of Nis, Serbia

*For correspondence: Email: vojkanmm_serbia@yahoo.com; Fax: +38118533014

Received: 7 March 2013 Revised accepted: 19 January 2014

Tropical Journal of Pharmaceutical Research, March 2014; 13(3): 417-422

http://dx.doi.org/10.4314/tjpr.v13i3.16

Abstract

Purpose: To develop an improved kinetic-spectrophotometric procedure for the determination of metronidazole (MNZ) in pharmaceutical formulations.

Methods: The method is based on oxidation reaction of MNZ by hydrogen peroxide in the presence of Fe(II) ions at pH 4.5 (acetate buffer). The reaction was monitored spectrophotometrically by measuring the rate of change of absorbance at 317nm.

Results: The optimum operating conditions for reagent concentrations and temperature were established. Linear calibration curve was obtained in the range of 85.77 – 513.45ng mL^-1 with standard deviation from 1.77 to 4.55 %-. The optimized conditions yielded a theoretical detection limit of 15.20 ng mL^-1 based on 3.3S_o criterion. Commonly used excipients and other additives such as talc, glucose, fructose, lactose, starch, magnesium stearate, microcrystalline cellulose and several ions were found not to have interference.

Conclusion: The developed method is sensitive, accurate and reproducible and could be used for routine analysis of metronidazole in pharmaceutical preparations.

Keywords: Metronidazole, Kinetic spectrometry, Validation, Pharmaceutical preparation.