In vitro Antiglycation and
Cross-Link Breaking Activities of Sri Lankan Low-Grown
Orthodox Orange Pekoe Grade Black Tea (Camellia
sinensis L)
Wanigasekara D
Ratnasooriya1*, Walimuni KSM Abeysekera2,
Tharaka BS Muthunayake1 and Chatura DT
Ratnasooriya1
1Department of Zoology,
University of Colombo, Colombo 03, 2Food
Technology Section, Industrial Technology Institute,
Colombo 07, Sri Lanka.
*For correspondence:
Email:
wdr@zoology.cmb.ac.lk; Tel:
+94112503399
Received: 1 February 2013
Revised accepted: 5
February 2014
Tropical
Journal of Pharmaceutical Research, April 2014;
13(4):
567-571
http://dx.doi.org/10.4314/tjpr.v13i4.12
Abstract
Purpose: To investigate the
antiglycation and cross-link breaking activities of Sri
Lankan low-grown orthodox Orange Pekoe grade black tea
(Camellia sinensis L)
Methods: Five concentrations (6.25,
12.5, 25.0, 50.0 or 100.0 µg/ml) of Black tea brew (BTB)
were made using Sri Lankan low-grown Orange Pekoe (O.P.)
grade tea. Antiglycation and advanced glycation end
products (AGEs) cross-link breaking activities of BTB as
well as the antiglycation activity of rutin were
determined in vitro on bovine serum albumin/glucose
system using fluorescence spectroscopy.
Results: BTB induced significant
(p<0.05) antiglycation activity (IC50, 19.04
± 5.18 µg/ml) and AGEs cross-link breaking activities
(IC50, 82.89 ± 3.44 µg/ml). These effects
were dose-dependent. Further, the antiglycation activity
of BTB was comparable to rutin, a well-known
antiglycation agent (IC50, 21.88 ± 2.82
µg/ml).
Conclusion: These results show that Sri
Lankan low-grown O.P. grade black tea possesses both
antiglycation and AGEs cross-link breaking activities in
vitro and thus provides scientific justification for the
use of black tea in Sri Lankan indigenous medicine for
the management of diabetic complications.
Keywords: Diabetic complications,
Antiglycation, Glycotoxin, Black tea, Cross-link
breakers, Sri Lankan tea, Orange Pekoe tea
