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Original Research Article
Antioxidative and
Hepatoprotective Activities of Deinoxanthin-Rich Extract
from Deinococcus radiodurans R1 against Carbon
Tetrachloride-Induced Liver Injury in Mice
Jianhui Cheng1,2,
Zuofa Zhang2, Zhiguo Zheng1,3,
Guoying Lv2, Liangyan Wang1, Bing
Tian1 and Yuejin Hua1*
1Institute of
Nuclear-Agricultural Sciences, Zhejiang University,
Kaixuan Road No. 268, 310029 Hangzhou, 2Department
of Horticulture, Zhejiang Academy of Agricultural
Sciences, Shiqiao Road No. 139, 310021 Hangzhou, 3Cancer
Research Institute, Zhejiang Cancer Hospital, Guangji
Road No. 139, 310020 Hangzhou, China
*For correspondence:
Email:
yjhua@zju.edu.cn; Tel: +86 571
86971703
Received: 18 October 2013
Revised accepted: 6
February 2014
Tropical
Journal of Pharmaceutical Research, April 2014;
13(4):
573-580
http://dx.doi.org/10.4314/tjpr.v13i4.13
Abstract
Purpose: To investigate the
antioxidant activity and hepatoprotective effect of
deinoxanthin-rich extract from Deinococcus radiodurans (EDR)
against CCl4-induced liver injury in mice.
Methods: The ethanol extract of EDR
was analyzed by liquid chromatography/mass spectrometry
(LC/MS), and its antioxidant activity was examined using
in vitro assays for reducing power, iron chelating
activity and lipid peroxidation. The extract was also
evaluated for its hepatoprotective activity against
carbon tetrachloride-induced liver injury in mice. The
activities of alanine aminotransferase (ALT), aspartate
aminotransferase (AST), alkaline phosphatase (ALP) in
serum, and catalase (CAT), superoxide dismutase (SOD)
and glutathione peroxidase (GSH-Px) in liver tissue, as
well as hepatic malondialdehyde (MDA) levels, were
measured to monitor liver injury. Damage to liver cells
was assessed by histology.
Results:
EDR displayed strong
reducing activity and lipid peroxidation inhibition
activity in vitro. Pretreatment with EDR (400 mg/kg
b.w.) significantly reduced activities of serum ALT, AST
and ALP, as well as hepatic MDA levels (p < 0.05), but
increased the activities of GSH-Px, CAT and SOD.
Histopathological assessment showed that liver tissue
damage was decreased by the protective effect of EDR.
Conclusion: The results show that EDR
can protect mice against CCl4-induced hepatic
damage by its antioxidant and free radical scavenging
activities.
Keywords: Antioxidant, Deinoxanthin,
Deinococcus radiodurans, Hepatoprotective, Carbon
tetrachloride, Liver damage |
