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Original Research Article


Chemical Composition and Insecticidal Activity of Essential Oil of Artemisia frigida Willd (Compositae) against Two Grain Storage Insects

 

Xin Chao Liu1, Yonglan Li2, Tiejuan Wang2, Qing Wang2 and Zhi Long Liu1*

1Department of Entomology, China Agricultural University, 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, 2College of Life Sciences and Technology, Inner Mongolia Normal University, Hohhot, Inner Mongolia 010022, China

 

*For correspondence: Email: zhilongliu@cau.edu.cn; Tel: +86-10-62732800; Fax: +86-10-62732800

 

Received: 18 October 2013                                                                   Revised accepted: 6 February 2014

  

Tropical Journal of Pharmaceutical Research, April 2014; 13(4): 587-592

http://dx.doi.org/10.4314/tjpr.v13i4.15   

Abstract

 

Purpose: To investigate the chemical composition and insecticidal activity of the essential oil of the aerial parts of Artemisia frigida against maize weevils (Sitophilus zeamais) and booklice (Liposcelis bostrychophila).

Methods: Steam distillation of A. frigida aerial parts was carried out in a Clavenger apparatus to extract its volatile oil content. Gas chromatography/mass spectrometric (GC/MS) analyses (HP-5MS column) of the essential oil were performed and its contact toxicity was determined using topical application and filter paper impregnation technique while its fumigant toxicity was evaluated using sealed-space method.

Results: A total of 32 components of the essential oil were identified. The principal compounds were cis-ρ-menth-2-en-1-ol (20.8%), 1,8-cineole (12.0%), borneol (10.2%), lavandulol (9.3%), camphor (6.9%), and bicyclogermacrene (5.5%). The oil exhibited contact toxicity against adult S. zeamais and L. bostrychophila with LC50 value of 17.97 µg/adult and 254.38 µg/cm2, respectively. The essential oils also possessed fumigant toxicity against S. zeamais and L. bostrychophila with LC50 value of 69.46 mg/L and 1.25 mg/L air.   

Conclusion: The study indicates that the essential oil of A. frigida has a potential to be developed to a natural fumigant/insecticide for the control of grain storage insects.

 

Keywords: Artemisia frigida, Sitophilus zeamais, Liposcelis bostrychophila, Insecticidal activity, Essential oil, Cis-ρ-Menth-2-en-1-ol; 1,8-Cineole

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