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Original Research Article
Development and Validation
of Improved Method for Fingerprint Analysis of
Rhizoma Chuanxiong by Capillary Zone Electrophoresis
with Ultraviolet-Diode Array Detection
Feng Huan Wei*, Qun Shen
and Fei Long Chen
Department of Chinese
Medicine Pharmaceutics, Southern Medical University,
Guangzhou 510515, China
*For correspondence:
Email:
awag7674@smu.edu.cn; Tel: +86
20 61648263; Fax: +86 20 61648244.
Received: 21 February 2014
Revised accepted: 21 April
2014
Tropical
Journal of Pharmaceutical Research, May 2014;
13(5): 773-778
http://dx.doi.org/10.4314/tjpr.v13i5.18
Abstract
Purpose: To develop and validate an
improved method by capillary zone electrophoresis with
photodiode array detection for the fingerprint analysis
of Ligusticum chuanxiong Hort. (Rhizoma Chuanxiong).
Methods: The optimum high
performance capillary electrophoresis (HPCE) conditions were
30 mM borax containing 5 %v/v acetonitrile at pH 9.6,
direct injection of water extract at 0.5 psi for 5s,
25kV running voltage at positive power, 20
℃ capillary temperature and 295 nm detection.
Between injections, capillary was rinsed using 0.1M
sodium hydroxide solution for 4 min, with HPCE-grade
water for 4 min and re-equilibrated with running buffer
for 2 min at 20 psi, moreover, the running buffer was
replaced after three consecutive injections.
Results: Baseline separation of most
of the 17 detectable peaks in the water extract could be
completed within 21 min.The relative standard deviations
(RSD) of precision, reproducibility, and stability were
< 2.79 % for retention time and 8.68 % for peak area of
the main peaks.
Conclusion: The developed method is
simple, fast, reliable and environment-friendly for the
fingerprint analysis of Rhizoma Chuanxiong water
extract.
Keywords: Capillary electrophoresis,
Rhizoma chuanxiong, Water extract, Fingerprint analysis,
Diode array |
