Tropical Journal of Pharmaceutical Research

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Original Research Article

A Prospective Cohort Study of IRS Genes Polymorphisms in Type 2 Diabetes Mellitus Patients during Severe/Acute Hyperglycemia Phase. 2: Association with Glycemic Control

Hasniza Zaman Huri^1,2*, Mohd Makmor-Bakry³, Rosnani Hashim⁴, Norlaila Mustafa⁵ and Wan Zurinah Wan Ngah⁶

¹Department of Pharmacy, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia, ²Clinical Investigation Centre, 13th Floor, Main Tower University of Malaya Medical Centre, 59100 Lembah Pantai, Kuala Lumpur, Malaysia, ³Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia, ⁴Faculty of Pharmacy, Cyberjaya University College of Medical Sciences Cyberjaya, Malaysia, ⁵Department of Medicine, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia, ⁶Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.

*For correspondence: Email: hasnizazh@um.edu.my, hasnizazh@ummc.edu.my; Tel: +603 79676659

Received: 15 October 2013 Revised accepted: 14 March 2014

Tropical Journal of Pharmaceutical Research, June 2014; 13(6): 903-906

http://dx.doi.org/10.4314/tjpr.v13i6.11

Abstract

Purpose: To investigate the genetic polymorphisms that may contribute to the worsening of glycemic control in type 2 diabetes mellitus (T2DM) with severe or acute hyperglycemia.

Methods: The prospective cohort study included 156 T2DM patients with severe or acute hyperglycemia from all medical wards of the National University of Malaysia Medical Centre (UKMMC) that were treated with insulin therapy. For the genetic association study, we used the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The glycemic control assessment during the acute phase was based on the degree to which the patient achieved the targeted glucose levels. The primary outcome was the influence of IRS gene on glycemic control in T2DM patients with severe/acute phase hyperglycemia.

Results: Insulin Receptor Substrate 1 (IRS1) (R2 = -0.023, p = 0.771) and Insulin Receptor Substrate 2 (IRS2) gene polymorphisms (R2 = -0.160, p = 0.046) were not associated with glycemic control in T2DM patients with severe/acute hyperglycemia.

Conclusion: The IRS1 and IRS2 genetic factors may not be significant genetic determinant for glycemic control in T2DM patients during severe/acute phase hyperglycemia.

Keywords: Insulin receptor substrate, Gene, Polymorphism, Diabetes, Insulin resistance, Hyperglycemia, IRS 1, IRS 2