|
|
Original Research Article
Preliminary Phytochemical
Screening, Acute Oral Toxicity and Anticonvulsant
Activity of the Berries of Solanum nigrum Linn
Hoang Le Son1*
and Phan Thi Hai Yen1
1School of Biotechnology,
International University – Vietnam National University,
Ho Chi Minh City, Vietnam
*For correspondence:
Email:
hlson@hcmiu.edu.vn; Tel: +84
908 311518; Fax: +84 8 37244271
Received: 15 November 2013 Revised accepted: 9 April
2014
Tropical Journal of Pharmaceutical Research, June
2014; 13(6): 907-912
http://dx.doi.org/10.4314/tjpr.v13i6.12
Abstract
Purpose: To
investigate the preliminary phytochemical properties,
acute oral toxicity and anticonvulsant activity of the
berries of Solanum nigrum Linn (S. nigrum)
Methods:
Phytochemicals from the ethanol berry extract were
screened by standard methods. Acute oral toxicity study
was conducted as per Organisation for Economic
Co-operation and Development (OECD) 425 guidelines while
anticonvulsant activity was evaluated against
pentylenetetrazole (PTZ)-induced seizure in mice. The
effect of the extract at dose levels of 50, 100, 200 and
300 mg/kg body weight was evaluated in an experimental
mice model, using phenobarbital as positive control (100
mg/kg p.o).
Results:
Phytochemical screening revealed that the berries of S.
nigrum contain carbohydrates, flavonoids, saponins,
tannins, alkaloids, phenols and steroids. The oral
median lethal dose of the extract was 3129 mg/kg body
weight. The extract significantly delayed the latency of
convulsion (p ˂ 0.05) in PTZ-induced seizure mice in at
the dose of 300 mg/kg p.o. The extract also reduced the
frequency of convulsion and provided up to 100 %
protection (300 mg/kg p.o) against death.
Conclusion: The results obtained in this study suggest
that the ethanol berry extract of Solanum nigrum is safe
and possesses anticonvulsant activity in PTZ-induced
seizure in mice.
Keywords: Solanum nigrum, Phytochemical,
Anticonvulsant, Pentylenetetrazole, Lethal dose, Acute
toxicity |
