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Original Research Article
Essential Oil Composition
and Anti-Inflammatory Activity of Salvia officinalis
L (Lamiaceae) in Murin Macrophages
Alev Tosun1*,
Salman Khan2, Yeong Shik Kim2,
Ángel Calín-Sánchez3,
Xhevit Hysenaj4
and Ángel Antonio Carbonell-Barrachina3
1Ankara University, Faculty of
Pharmacy, Department of Pharmacognosy, 06100, Tandoğan,
Ankara, Turkey, 2Natural Product Research
Institute, College of Pharmacy, Seoul National
University, Seoul, 151-742, South Korea, 3Departamento
Tecnología Agroalimentaria, Universidad Miguel Hernández
de Elche, Carretera de Beniel, 03312 Orihuela, Alicante,
Spain, 4Xherdo Ltd., Medicinal Plants and
Essential Oils, Nikolla Lena, No.138, Tirana, Albania
*For correspondence:
Email:
pharmacogalev@gmail.com; Tel:
+90 312 203 30 89; Fax: +90 312 213 10 81
Received: 13 May 2013
Revised accepted: 2 April
2014
Tropical Journal of Pharmaceutical Research, June
2014; 13(6): 937-942
http://dx.doi.org/10.4314/tjpr.v13i6.16
Abstract
Purpose: Sage, Salvia officinalis L
(Lamiaceae), is widely cultivated medicinal plant for
its economic importance and large content of bioactive
components; therefore, in the present study, the active
components (volatile compounds) and the
anti-inflammatory effect of S. officinalis have been
investigated.
Methods: Salvia officinalis samples
were collected from northern and southern Albania,
respectively. The crushed leaves were subjected to
hydro-distillation, and the essential oils analyzed by
gas chromatography GC/FID (for quantification of
volatiles) and gas chromatography/mass spectroscopy
(GC/MS) for identification.
Results: α-Thujone (30.7 %), camphor
(26.6 %) and 1, 8-cineole (14.7 %) were the major
components of the oil from northern Albania, while
camphor (43.8 %), α-thujone (15.9 %), camphene (8.5 %)
and 1,8-cineole (8.4 %) were the predominant compounds
in the sample from southern Albania. The results of the
anti-inflammatory tests on these essential oils using
murine macrophages indicate that both oils significantly
(p < 0.05) reduced nitric oxide (NO), and nuclear kappa
B (NF-κB) production in RAW 264.7 cells.
Conclusion: The results indicate that
NO and NF-κB production in RAW 264.7 cells are greatly
decreased by the essential oil of Albanian sage. Thus,
the biological properties of sage oil can be attributed
to the components of the oil.
Keywords: Salvia officinalis, Sage
oil, Camphor, Camphene, Lamiaceae, Cineole, Nuclear
kappa B, Nitric oxide, α-Thujone, Volatile compound |
