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Original Research Article
In vitro Antitumor Activities of
Platycarya strobilacea Sieb et Zucc
Infructescence Extracts
Liangliang Zhang1,2*,
Yongmei Wang1,2 and Man Xu1
1Institute of Chemical
Industry of Forest Products, Chinese Academy of
Forestry, National Engineering Lab. for Biomass Chemical
Utilization, Key and Open Lab. of Forest Chemical
Engineering, State Forestry Administration, Key Lab. of
Biomass Energy and Material, Jiangsu Province, Nanjing
210042, 2Institute of New Technology of
Forestry, Chinese Academy of Forestry, Beijing 100091,
China
*For correspondence:
Email:
zhll20086@163.com; Tel: +86 25
85482463; Fax: +86 25 85482463
Received: 30 August 2013
Revised accepted: 15 March
2014
Tropical Journal of Pharmaceutical Research, June
2014; 13(6): 849-854
http://dx.doi.org/10.4314/tjpr.v13i6.4
Abstract
Purpose: To
evaluate the antitumor activities of Platycarya
strobilacea infructescence extracts in A549, HepG2,
SH-SY-5Y, HCT116, and U2OS-NKFB cell lines.
Methods: The
total amount of phenolics in P. strobilacea
infructescence based on three solvent extracts
(methanol, ethyl acetate and water) was measured using
Folin-Ciocalteu reagent method. The cytotoxic effect of
the various solvent extracts and tannins on five
cancerous cell lines (A549, HepG2, SH-SY-5Y, HCT116, and
U2OS-NKFB) were assayed using
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide [MTT].
Results:
Ethyl acetate extract had the highest phenolic content
(725.02 mg GAE/g), followed by methanol extract (627.64
mg GAE/g). Among the extracts tested, ethyl acetate
extract had effective anticancer activity against A549,
HepG2, HCT116 and U2OS-NKFB cells with IC50 values of 40.1, 42.6, 40.1 and
73.4 µg/mL, respectively. Methanol extract had the
strongest cytotoxic effect on SH-SY-5Y cell with IC50 value of 31.2 µg/mL. The number
of surviving cancerous cells decreased as the
concentration of the ethyl acetate and methanol extracts
increased.
Conclusion: The study confirms the antitumor activities
of ethyl acetate and methanol extracts of P. strobilacea
infructescence. Thus, the plant extracts constitute
potential therapeutic materials that require further
studies and development.
Keywords: Platycarya strobilacea, Antitumor, Phenolic
compounds, Tannins, MTT assay |
