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Original Research Article
Formulation of
Thermosensitive Hydrogel Containing Cyclodextrin for
Controlled Drug Delivery of Camptothecin
Swatantra Kumar Singh
Kushwaha1*, Awani Kumar Rai1 and
Satyawan Singh2
1Pranveer Singh Institute of
Technology, Kanpur, 2Saroj Institute of
Technology & Management, Lucknow, India
*For correspondence:
Email:
swatantrakushwaha@yahoo.co.in;
Tel: +91-9450560321
Received: 30 August 2013
Revised accepted: 1 May
2014
Tropical
Journal of Pharmaceutical Research, July 2014;
13(7): 1007-1012
http://dx.doi.org/10.4314/tjpr.v13i7.1
Abstract
Purpose: To
formulate and evaluate temperature-sensitive,
controlled-release camptothecin hydrogel for anticancer
drug delivery.
Method:
Temperature-sensitive hydrogel based on chitosan/β-glycerophosphate
(β-GP)/β-cyclodextrin (β-CD) was prepared by
crosslinking method. The formulations were characterized
by Fourier transform infrared spectroscopy (FTIR), x-ray
diffraction (XRD), gelation time, and viscometry, as
well as for controlled release. The formulation,
containing camptothecin, was studied by MTT assay on
tumor cell MCF-7. The effectiveness of treatment was
measured in terms of controlled tumor growth inhibition
(TGI).
Results: The
hydrogel formulation showed good properties in terms of
pH, gelation, viscosity and in-vitro release. The
gelation temperature and viscosity of the formulation
was optimum. Camptothecin (CPT) released from the
hydrogel (TF8) over 8 h in pH 7.4 buffer ranged from
38.97 - 92.5 %, and varied according to the composition
of the hydrogels. Release of camptothecin was lowest
from preparations without cyclodextrins. Tumor growth
inhibition activity of CPT in MCF-7 cell was highest for
the formulation containing 1 % chitosan, 8 % β-GP and 1
% β-CD while no inhibition was observed for the blank
temperature sensitive hydrogel formulation.
Conclusion: These
formulations are a promising and more effective delivery
system that can be developed to serve as an alternative
to the conventional system for anticancer drug delivery.
Keywords: Hydrogel, Chitosan, β-Glycerophosphate, β-Cyclodextrin,
Camptothecin, MCF-7 cell line |
