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Original Research Article
Allicin Protects against
Lipopolysaccharide-Induced Acute Lung Injury by
Up-Regulation of Claudin-4
Yue-liang Zheng, Wen-wei
Cai, Guang-zhao Yan, Yuan-zhan Xu and Mei-qi Zhang*
Department of Emergency,
Zhejiang Provincial People's Hospital, Hangzhou 310014,
China
*For correspondence:
Email:
meiqizhang1208@153.com; Tel:
+86-0571-85893631
Received: 8 January 2014
Revised accepted: 31 May
2014
Tropical
Journal of Pharmaceutical Research, July 2014;
13(7): 1063-1069
http://dx.doi.org/10.4314/tjpr.v13i7.8
Abstract
Purpose: To
investigate the effect of allicin, an active component
of garlic, on lipopolysaccharide (LPS)-induced acute
lung injury.
Methods:
Wistar rats were subjected to LPS intravenous injection
with or without allicin treatment to induce acute lung
injury (ALI) model. Also, A549 cells were stimulated
with LPS in the presence and absence of allicin. HE
staining was used to detect pathological changes in lung
tissues. Enzyme-linked immunosorbent assay (ELISA) was
performed to measure cytokine content. Cell viability
was measured by CCK-8 and EdU incorporation assay. Genes
expression was determined by real time polymerase chain
reaction (PCR) and Western blot. Flow cytometry was
applied to measure cell apoptosis.
Results: In
vivo data showed that pulmonary edema, inflammatory
cytokines expression and pathological changes were
significantly attenuated in LPS-induced ALI after
treatment with allicin (p < 0.05) while in vitro results
indicate that allicin administration significantly
improved the A549 cell viability in a dose-dependent
manner as measured by CCK-8 and EdU incorporation assay.
Besides, flow cytometry analysis showed that cell
apoptosis rate was significantly reduced in a
concentration-dependent manner after allicin injection
(30.3 vs. 11.8 %, p < 0.05). At the molecular level,
allicin treatment dose-dependently up-regulated
claudin-4 expression both in vivo and in vitro (p <
0.05).
Conclusion: The findings indicate that allicin can
protect against LPS-induced ALI in vivo and in vitro
probably by up-regulation of claudin-4 expression.
Keywords: Allicin, Acute lung injury,
Lipopolysaccharide, Claudin-4, Up-regulation, Pulmonary
edema, Inflammatory cytukines |
