Comparative Antioxidant,
Antiproliferative and Apoptotic Effects of Ilex
laurina and Ilex paraguariensis on Colon
Cancer Cells
Juan M Pérez1,
Maria E Maldonado2, Benjamín A Rojano3,
Fernando Alzate4, Jairo Sáez1 and
Wilson Cardona1*
1Grupo de Química de Plantas
Colombianas, Instituto de Química, 2Grupo de
Impacto de los Componentes en la Salud, Escuela de
Nutrición y Dietética, Universidad de Antioquia, A.A.
1226 Medellín, 3Grupo Ciencia de los
Alimentos, Facultad de Ciencias, Universidad Nacional de
Colombia. A.A. 1027 Medellin, 4Grupo de
Estudios Botánicos, Instituto de Biología, Facultad de
Ciencias Exactas y Naturales, Universidad de Antioquia,
A.A. 1226 Medellín, Colombia
*For correspondence:
Email:
wilson.cardona1@udea.edu.co;
Tel: +574-2195653; Fax: +57-42330120
Received: 30 August 2013
Revised accepted: 17 June
2014
Tropical
Journal of Pharmaceutical Research, August 2014; 13(8): 1279-1286
Abstract
Purpose: To determine and compare
the antioxidant, antiproliferative and apoptotic effects
of leaf infusions of Ilex laurina and Ilex
paraguariensis in colon cancer cells.
Methods: Antioxidant activity was
determined by ORAC (Oxygen Radical Absorbance Capacity)
and FRAP (Ferric Reducing Antioxidant Power). Cytotoxic
and antiproliferative effects were analyzed using MTT
((3-(4, 5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium
bromide) and sulfhorodamine-B respectively. Cell death
and apoptosis of human colon adenocarcinoma cells SW480
and their metastatic-derived SW620 cells, were analyzed
by flow cytometry using propidium iodide and Annexin-V.
Results: Although their flavonoid
levels were similar, I. laurina infusion contained 2.2
and 4.4 times higher amounts of total phenolic and
caffeoyl derivatives, respectively, than I.
paraguariensis. FRAP and ORAC values for I. laurina
infusion were 1.6 and 2.0 more active than I.
paraguariensis. Both plant infusions inhibited viability
and cell growth of SW480 and SW620 cells. These results
may be associated to cell cycle-arrest and apoptosis
because of the comparable increase of hypodiploid and
annexin-V positive colon cancer cells.
Conclusion: These data highlight the
antioxidant and promising anticancer activities of I.
laurina and Ilex paraguariensis.
Keywords: Ilex laurina, Ilex
paraguariensis, Antioxidant, Antiproliferative,
Apoptosis, Colon cancer
