|
|
Original Research Article
Antimicrobial and
Antioxidant Activities of the Essential Oils of Some
Aromatic Medicinal Plants (Pulicaria inuloides-Asteraceae
and Ocimum forskolei-Lamiaceae)
Nabil Qaid M Al-Hajj1,2,
Hong Xing Wang1*, Chaoyang Ma1,
Zaixiang Lou1, Mohanad Bashari1
and Riyadh Thabit1
1State Key Laboratory of Food
Science and Technology, School of Food Science and
Technology, Jiangnan University, Wuxi 214122, PR China,
2Marine Science and Biological Research
Authority, Aden, PO Box 1231, Aden, Yemen
*For correspondence:
Email:
whx1964@126.com; Tel:
+8651085917795; Fax: +8651085876799
Received: 24 March 2014
Revised accepted: 11 July
2014
Tropical
Journal of Pharmaceutical Research, August 2014; 13(8): 1287-1293
http://dx.doi.org/10.4314/tjpr.v13i8.13
Abstract
Purpose: To determine the
antimicrobial and antioxidant activities of Pulicaria
inuloides and Ocimum forskolei essential oils.
Methods: Steam distillation of the
aerial parts of P. inuloides and O. forskolei was
performed using a Clevenger apparatus. Essential oils
were analyzed by gas chromatography–mass spectrometry.
Total phenolic content and antioxidant activities were
determined by 2,2-diphenyl-1,1-picrylhydrazyl (DPPH) and
β-carotene bleaching assays. Disc diffusion and
microtiter broth microdilution assays were employed to
determine antimicrobial activity.
Results: The chemical compounds in
P. inuloides essential oil include 2-cyclohexen-1-one,
2-methyl-5-(1-methyl) (55.1 %) and benzene, methyl-
(20.6 %). The major components identified in O.
forskolei essential oil included bicyclo [3.1.1]
hept-2-ene,2, (22.4 %) and naphthalene 1,2,3,4,4a,5,6,
(19.3 %). P. inuloides showed a higher total phenol
content than O. forskolei (144 ± 5.32 vs. 54.6 ± 30 mg
GAE/g extract), higher antioxidant activity (92.92 ±
0.10 % vs. 26.76 ± 0.11 % scavenging activity; IC50, 4.5
± 0.05 vs. 73.03 ± 0.05) and β-carotene bleaching (90.77
± 0.21 % vs. 41.03 ± 6.35 % inhibition). P. inuloides
essential oil inhibited all tested microorganisms except
Salmonella typhimurium and Shigella dysenteriae with a
minimum inhibitory concentration (MIC) of 3.0 μg/mL
against Escherichia coli. O. forskolei essential oil
inhibited only Candida albicans.
Conclusion: P. inuloides essential oil
possesses significant antioxidant and antimicrobial
activities.
Keywords: Essential oil; Phenolic
content; Antioxidant; Antimicrobial activity, Pulicaria
inuloides, Ocimum forskolei, Salmonella typhimurium,
Shigella dysenteriae |
