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Original Research Article
Evaluation of Gentamicin-Entrapped
Solid Lipid Microparticles Formulated with a
Biodegradable Homolipid from Capra hircus
FC Kenechukwu1*,
CE Umeyor2, MA Momoh1, JDN Ogbonna1,
SA Chime3, PO Nnamani1 and AA
Attama1
1Department of Pharmaceutics,
University of Nigeria, Nsukka 410001, 2Department
of Pharmaceutics and Pharmaceutical Technology, Nnamdi
Azikiwe University, Awka, 3Department of
Pharmaceutical Technology and Industrial Pharmacy,
University of Nigeria, Nsukka 410001, Nigeria
*For correspondence:
Email:
chimafrankduff@yahoo.com;
frankline.kenechukwu@unn.edu.ng;
Tel: +234 8038362638; Fax: +234-42-771709
Received: 5 October 2013
Revised accepted: 15 June
2014
Tropical
Journal of Pharmaceutical Research, August 2014; 13(8): 1199-1205
http://dx.doi.org/10.4314/tjpr.v13i8.2
Abstract
Purpose: To formulate solidified
reverse micellar solutions (SRMS)-based solid lipid
microparticles (SLMs) using homolipid from Capra hircus,
and evaluate its suitability for the delivery of
gentamicin.
Methods: SLMs were formulated by
melt-emulsification using SRMS (15 % w/w Phospholipon®
90G in 35 % w/w Capra hircus), PEG 4000 and gentamicin
(1.0, 2.0 and 3.0 % w/w), and characterized with respect
to size, morphology, encapsulation efficiency (EE) and
pH-dependent stability. In vitro release of gentamicin
from the SLMs was performed in phosphate buffer (pH 7.4)
while bioevaluation was carried out using clinical
isolates of Pseudomonas aeruginosa and Staphylococcus
aureus.
Results: Stable and discrete SLMs of
size range 1.47 ± 0.02 to 3.55 ± 0.09 µm were obtained.
The SLMs showed a biphasic pattern of drug release and
exhibited time-dependent and capacity-limited
bioactivity. Overall, SLMs containing 2 % w/w SRMS, 3 %
w/w gentamicin and PEG 4000 entrapped the highest
amount of drug, released 99 % of drug and gave the
highest inhibitory zone diameter (IZD) against the
organisms within 420 min, while plain gentamicin gave
the least.
Conclusion: SRMS-based SLMs prepared
with homolipid from Capra hircus offers a suitable
delivery system for gentamicin.
Keywords: Solid lipid microparticles,
Gentamicin, Capra hircus, Phospholipon® 90 G, Solidified
reverse micellar solution |
