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Original Research Article
Evaluation of Wound
Healing Effect of Punica granatum L Peel Extract
on Deep Second-Degree Burns in Rats
Ke Ma1, Mindong
Du2, Mingde Liao1*, Shihai Chen1,
Guoqian Yin1, Qingfeng Liu1, Qiang
Wei1 and Gang Qin2
1Department of Plastic &
Cosmetic Surgery, The First Affiliated Hospital of
Guangxi Medical University, Nanning, 530021, 2Department
of Orthopaedics, The First Affiliated Hospital of
Guangxi University of Chinese Medicine, Nanning, 530023
Guangxi, China
*For correspondence:
Email:
make133494@163.com; Tel: +86 0771
5356512
Received: 15 August 2014
Revised accepted: 1
December 2014
Tropical Journal of Pharmaceutical Research,
January 2015;
14(1):
73-78
http://dx.doi.org/10.4314/tjpr.v14i1.11
Abstract
Purpose: To investigate the healing
effect of Punica granatum L. peel (Pomegranate husk)
extract (PHE) on deep second-degree burns in rats.
Methods: Male Wistar rats, weighing
220 – 240 g, were subjected to deep second-degree skin
burns by electrical scald instrument. The animals were
divided into three groups as follows: (1) deep
second-degree burn model (control) group, (2) burns
model treated with 1 % silver sulfadiazine (SSD) group,
and (3) burns model treated with PHE group. On days 3,
7, 14 and 21, animal weight, wound area as well as
histopathological features of skin were evaluated for
all the groups. Minimum inhibitory concentration (MIC)
of PHE on Staphyloccocus aureus, Pseudomonas aeruginosa
and Escherichia coli were also assessed separately.
Results: On day 21, the mean wound
area of PHE treatment group (0.21 ± 0.07 cm2)
and SSD treatment group (1.15 ± 0.1 cm2) were
significantly smaller than that of control rats (2.42 ±
0.2 cm2, p < 0.01) respectively. Histological
results indicate that inflammatory cells disappeared
substantially and were replaced by new granulation
tissue in PHE treatment or SSD treatment group, while
that of the control rats still showed severe
inflammatory cell infiltration. Antibacterial test
revealed that the MIC of PHE on Staphyloccocus aureus,
Pseudomonas aeruginosa and Escherichia coli was 6.25,
12.5 and 6.25 mg•mL-1, respectively.
Conclusion: The results reveal that PHE
is an effective and promising medicinal herb for the
management of deep second degree burns.
Keywords: Punica
granatum L. peel, Burns, Wound area, Healing,
Antimicrobial activity |
