Tropical Journal of Pharmaceutical Research

Indexed by Science Citation Index (SciSearch), International Pharmaceutical Abstract, Chemical Abstracts, Embase, Index Copernicus, EBSCO, African Index Medicus, JournalSeek, Journal Citation Reports/Science Edition, Directory of Open Access Journals (DOAJ), African Journal Online, Bioline International, Open-J-Gate

ISSN: 1596-5996 (print); 1596-9827 (electronic)-

Home | Back Issues | Current Issue | Review manuscript | Submit manuscript


	This Article
	Abstract
	Full-Text (PDF)
	Table of contents
	Comments
	Letters
	Comments to Editor

	e-mail Alert
	Sign Up

Original Research Article

Preparation and Characterization of Nanoliposomal Beta-Cryptoxanthin and its Effect on Proliferation and Apoptosis in Human Leukemia Cell Line K562

Amir Gharib¹*, Zohreh Faezizadeh¹ and Masoud Godarzee²

¹Department of Laboratory Sciences, Borujerd Branch, Islamic Azad University, Borujerd, Iran, ²Department of Biology, Borujerd Branch, Islamic Azad University, Borujerd, Iran

*For correspondence: Email: amirgharib@gmail.com; Tel: +986623500201; Fax: +986624453013

Received: 22 October 2014 Revised accepted: 16 January 2015

Tropical Journal of Pharmaceutical Research, February 2015; 14(2): 187-194

http://dx.doi.org/10.4314/tjpr.v14i2.1

Abstract

Purpose: To prepare beta-cryptoxanthin-loaded nanoliposomes and evaluate their anti-proliferative activity in leukemia K562 cell line, compared to free beta-cryptoxanthin.

Methods: Beta-cryptoxanthin-loaded nanoliposomes were prepared by extrusion method. Morphological characterization of the nanoliposomes was performed by cryo-transmission electron microscopy (cryo-TEM). The anti-proliferation effect of beta-cryptoxanthin (BC) in free and liposomal forms on K562 cell line was studied using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide assay. Apoptotic activity, following treatment with beta-cryptoxanthin in the free and liposomal forms, was detected using flow cytometry.

Results: Entrapment efficiency of beta-cryptoxanthin was 86.3 % ± 1.0. Cryo-TEM analysis revealed that the nanoliposomes have spherical shapes. In all conditions, beta-cryptoxanthin-loaded nanoliposomes exhibited greater anti-proliferative activity than than the free beta-cryptoxanthin (p < 0.001). Furthermore, in the presence of beta-cryptoxanthin-loaded nanoliposomes, the proportion of apoptotic cells was higher for free beta-cryptoxanthin (p < 0.001).

Conclusion: The data obtained indicate that beta-cryptoxanthin, especially in the liposomal form, inhibits the growth of K562 cells and may therefore provide a basis for the development of leukemia therapies.

Keywords: Beta-cryptoxanthin, Nanoliposome, Anti-proliferative, Apoptosis, Flow cytometry, Leukemia