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Original Research Article
Anti-Hyperprolactinemic
Effect of Formula Malt Decoction, a Chinese Herbal
Cocktail
Ming-xia Li1,
Hong Liu2, Yan Li3, Fei Wang4,
Pei-rong Zhang5 and Ping Zang6*
1College of Nursing, Shandong
University of Traditional Chinese Medicine, Jinan,
250355, 2Department of Business Development,
Affiliated Hospital of Weifang Medical University,
3ICU, Weifang Traditional Chinese Hospital, 4Department
of Neurosurgery, 5ICU, 6Public
Health Management Office, Affiliated Hospital of Weifang
Medical University, Weifang, 261031, Shandong, China
*For correspondence:
Email:
zangping133494@163.com; Tel:
+86 05368536155
Received: 29 September 2014 Revised accepted: 17 January
2015
Tropical Journal of Pharmaceutical Research,
February 2015;
14(2):
263-269
http://dx.doi.org/10.4314/tjpr.v14i2.11
Abstract
Purpose: To investigate the anti-hyperprolactinemic
activity of Formula Malt Decoction (FMD), a Chinese
herbal mixture.
Methods: The effect of FMD on serum
prolactin (PRL), estradiol (E2), progesterone (PGN),
follicle stimulating hormone (FSH) and luteinizing
hormone (LH) levels were investigated in
hyperprolactinemic (hyperPRL) rats. The effect of FMD on
PRL secretion, dopamine D2 receptor and dopamine
transporters (DAT) were studied in MMQ cells derived
from rat pituitary adenoma cells, GH3 cells derived from
rat pituitary lactotropic tumoral cells and PC12 cells
from rat pheochromocytoma, respectively.
Results: Compared with the model
group, a high dose of FMD (11.6 g/kg body weight) and
medium dose of FMD (5.8 g/kg body weight) reduced PRL
level of hyperPRL rats effectively. In MMQ cells,
treatment with 5 mg/ml FMD (p < 0.01) or 10 mg/mL FMD (p
< 0.01) significantly suppressed PRL secretion and
synthesis at 24 h compared with controls (p < 0.01).
Consistent with D2- action, FMD did not affect PRL in
rat pituitary lactotropic tumor-derived GH3 cells that
lack the D2 receptor expression compared with controls,
but 8 mg/mL FMD significantly increased the expression
of D2 receptors (p < 0.01) and DAT (p < 0.01) in PC12
cells.
Conclusion: FMD shows anti-hyperprolactinemia
activity via dopamine D2 receptor.
Keywords: Formula Malt Decoction,
Hyperprolactinemia, Dopamine D2 Receptor, Prolactin,
Estradiol, Progesterone follicle stimulating hormone,
Luteinizing hormone |
