Tropical Journal of Pharmaceutical Research | ||||||||||||||
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In Vitro Antimicrobial Activity of Stevia Rebaudiana Bertoni Leaves Manish
B. Tadhani
and Rema Subhash Food Biotechnology Laboratory, Post Graduate Department of Home Science, Sardar Patel University, Vallabh Vidyanagar-388 120 Gujarat, INDIA
Tropical Journal of Pharmaceutical Research 2006; 5(1): 557-560 ISSN: 1596-5996
Abstract
Purpose:
The aim of the present study was to investigate antimicrobial activity of the
various extracts of Stevia rebaudiana leaves. Method:
Stevia rebaudiana Bertoni leaves were extracted in water, methanol, ethyl
acetate and hexane and their antimicrobial activities were examined against few
selected microorganisms including B. subtilis, S.
aureus, M. luteus, S. marcenscens, P. aeruginosa, B. megaterium, E. coli, P.
vulgaris, Yeast, A.
niger and R. oligoporus using cup plate method. Results:
Water extract of Stevia leaf showed activity against B. subtilis and
S. aureus only. Methanol extract gave the highest zone of inhibition against P.
aeruginosa whereas minimum zone of inhibition was found against S. aureus and
yeast. B. megaterium and yeast were found to be highly susceptible towards ethyl
acetate and hexane extracts, respectively whereas A. niger and B. subtilis were
found to be least susceptible against ethyl acetate and hexane extracts,
respectively. Hexane extract showed the highest activity against yeast among the
tested microorganisms. Conclusion:
The study confirms the possible antimicrobial potentiality of the leaf
extract of Stevia rebaudiana. To whom correspondence should be addressed: E-mail: manish_fbt@yahoo.co.in Tel: 91 2692 226878 Ext. 23
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Last updated: August 30, 2006 |