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Original
Research Article
Anti-Cancer Properties of Diethylether Extract of Wood
from Sukun (Artocarpus altilis) in Human Breast
Cancer (T47D) Cells
Enos Tangke Arung1,2,
Britanto Dani Wicaksono1, Yohana Ayupriyanti
Handoko1, Irawan Wijaya Kusuma2,
Dina Yulia1 and Ferry Sandra1*
1Stem
Cell and Cancer Institute, Jalan Ahmad Yani no. 2
Pulomas, Jakarta, 13210, 2Wood Chemistry
Laboratory, Forest Product Department, Forestry Faculty,
Mulawarman University; Jalan KH. Dewantara, Kampus Gn.
Kelua, Samarinda, East Kalimantan 75123, Indonesia.
*Corresponding
author: E-mail: fsandra@sci-indonesia.org
Tel: +62-21-47860173; Fax: +62-21-47860180
Received: 6 February
2009
Revised accepted: 28 April 2009
Tropical
Journal of Pharmaceutical Research, August 2009; 8(4):
317-324
Abstract
Purpose:
To evaluate the anti-cancer properties of the
diethylether extract of Sukun (Artocarpus altilis) wood.
Methods:
The extract was tested in human T47D breast cancer cells
and examined for its effect on cell viability, nuclear
morphology and sub-G1 formation. Cell viability was
determined by microculture tetrazolium technique (MTT),
nuclear morphology investigated using
4’-6-diamidino-2-phenylindole (DAPI) staining technique,
and cell cycle progression monitored by sub-G1 apoptosis
assay using flow cytometry.
Results:
The results showed decreasing cell viability in a
concentration-dependent manner. Altered cell morphology
after treatment with the extract demonstrated that cells
experienced apoptosis. Cell cycle analysis indicated
that the number of cells in sub-G1 phase rose with
increasing concentrations of the extract.
Conclusion:
The data demonstrate that Sukun wood extract induced
apoptosis and sub-G1 phase formation in breast cancer
(T47D) cells, and therefore, has a potential as an
anti-cancer agent.
Keywords:
Artocarpus altili; Diethylether extract; T47D cells;
Anti-cancer; Apoptosis; Sub-G1 formation. |
