Extractive Spectrophotometric Determination of
Omeprazole in Pharmaceutical Preparations
Amol Bhandage1, Ashok Bhosale1, Ashok Kasture2 and
Vijaya Popatrao Godse1*
1Department
of Pharmaceutical Analysis, S. G. R. S. College of
Pharmacy, Saswad, Pune. (MS), 412301, 2Department
of Pharmaceutical Sciences, Nagpur University, Nagpur,
India.
*Corresponding author:
E-mail:
vijayagodse2002@gmail.com Tel:
+91-9923265133
Received: 5 March
2009
Revised accepted: 12 July 2009
Tropical
Journal of Pharmaceutical Research, October 2009; 8(5):
449-454
Abstract
Purpose:
To
develop a simple, rapid and selective method for the
extractive spectrophotometric determination of
omeprazole using acidic dyes.
Methods:
Extractive spectrophotometric determination of
omeprazole was developed using acidic dyes - bromophenol
blue and orange G - as ion-pairing agents in aqueous
medium (pH 7.0 and 6.0, respectively). The ion pair
chromogen formed, which was extracted with chloroform,
was measured quantitatively at 408 nm and 508 nm,
respectively. The developed method was used to analyse
commercial omeprazole tablets.
Results:
Using bromophenol blue and orange G dyes, the ion-paired
formed obeyed Beer’s law in the ranges 5 - 30 µg/ml and
50 - 250 µg/ml at 408nm and 503nm, respectively, with
molar absorptivities of 1.712 × 103 L mol-1
cm-1 and 2.095 × 103 L mol-1
cm-1, respectively, for omeprazole,. The
purity of omeprazole obtained was 98.1±0.9 and 99.7±0.3,
respectively. Standard deviation (S.D.), % relative
standard deviation (% R.S.D.) and standard error were
0.001 - 0.013, 0.94 - 1.07 % and 4 × 10-4,
respectively. The complexes formed were stable for
approx. 3 h.
Conclusion:
Recovery studies gave satisfactory results indicating
that none of the major additives/excipients interferred
with the assay method. Therefore, a simple, rapid and
selective method was developed for extractive
spectrophotometric determination of omeprazole. This
method may be useful for routine laboratory analysis of
omeprazole.
Keywords:
Extractive spectrophotometry, Omeprazole, Bromophenol
blue, Orange G.
