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Research
Article
Effect of Cedrus
deodara (Pinaceae) Root Oil on the Histopathology of
Rat Liver and Kidney
Rehana Parveen1,
Muhammad A Azmi2*,
Syed Naeemul H Naqvi1,
Syed M Mahmood3 and
Ijaz H Zaidi4
1Department
of Pharmacology, Institute of Pharmaceutical Sciences,
2Department
of Physiology, Baqai Medical University, 51 Deh Tor,
Gadap Road, Near Toll Plaza, Super Highway, P.O. Box
2407, Karachi-74600,
3Department
of Oncology, Agha Khan University Hospital, National
Stadium Road,
4Department
of Casualty, P.N.S. Shifa Hospital, DHA, Phase 2,
Karachi, Pakistan.
*Corresponding author:
E-mail:
azmiahmed@hotmail.com Tel:
+9221-4410434; Fax: +9221-4410439
Received: 15 October
2009
Revised
accepted: 25 February 2010
Tropical
Journal of Pharmaceutical Research, April 2010;
9(2):
127-133
Abstract
Purpose:
To evaluate the
effect of Cedrus deodara (Pinaceae) root oil, used
traditionally for the treatment of various ailments, on
the histopathology of the liver and kidney of Wistar
albino rats.
Methods:
The oil was
extracted from the plant root by dry destructive
distillation method and administered orally at doses of
0.5 ml and 2.5 ml per rat. Each animal group was
comprised of 30 rats. Treated and untreated group
of rats were sacrificed and organs (liver and kidney)
were taken out for tissue processing and staining with
haematoxylin and eosin according to the method of
Bancroft and Stevens. Microscopic examination was then
carried out to observe any pathological changes in the
animals.
Results:
The root oil
produced atrophic changes and haemolysis in the
glomerular region as well as shrinkage and shedding of
glomerular epithelium in the kidney tissues. Marked
dilation of the central vein and congested blood vessels
in the rat liver at both doses tested was also seen.
Conclusion:
The root oil of
C. deodara (Pinaceae), at the doses administered,
produced some adverse effects on the tissues tested but
these effects were not lethal. Some toxic changes in the
tissues of liver and kidney were also found.
Keywords:
Histopathology, Cedrus deodara root oil, Liver, Kidney,
Albino rats. |
