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Research Article

Isolation and Morphological Characterization of Antibiotic Producing Actinomycetes

Nanjwade Basavaraj K¹*, S Chandrashekhara², Ali M Shamarez¹, Prakash S Goudanavar² and Fakirappa V Manvi¹

¹KLES’s College of Pharmaceutical Sciences, K.L.E. University, Nehru Nagar, Belgaum, Karnataka,²KLES’s College of Pharmaceutical Sciences, Nipani, Karnataka, India

*Corresponding author: E-mail: bknanjwade@yahoo.co.in Tel: +91-8312471399 Fax: +91-8312472387

Received: 12 March 2009 Revised accepted: 21 April 2010

Tropical Journal of Pharmaceutical Research, June 2010; 9(3): 231-236

Abstract

Purpose: To isolate and characterize antibiotic producing actinomycetes from soil samples in Belgaum, Karnataka, India.

Methods: Crowded plate technique was used for the isolation of actinomycetes in media such as soybean – casein digest medium and actinomycetes isolation agar. The morphological and cultural characterization of one of the selected strains, designated A-4, was performed as per International Streptomycete Project (ISP).

Results: Morphological and cultural studies showed that A-4 belonged to the Actinomycete genus. The morphological and cultural characteristics of the A-4 mutant showed cellular and aerial growth as well as soluble pigment formation in various ISP media.

Conclusion: Findings from this investigation revealed that the selected strain, A-4, is an actinomycete.

Keywords: Actinomycete, Antibiotic, MIC, Crowded plate technique, Morphology.