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Research
Article
Spectrophotometric Determination of Cilostazol in Tablet
Dosage
Form
Pawan Kumar
Basniwal1*,
Vinesh Kumar1, Prabhat Kumar Shrivastava2
and Deepti Jain3
1LBS College of Pharmacy, Tilak Nagar, Jaipur – 302004, Rajasthan,
2Department of Pharmaceutics, Banaras Hindu
University, Banaras, UP, 3School of
Pharmaceutical Sciences, Rajiv Gandhi Technical
University, Bhopal (MP), India
*Corresponding author:
E-mail:
pawanbasniwal@gmail.com Tel:
+91-9414788171 (mob).
Received: 8 January
2010 Revised
accepted: 21 July 2010
Tropical
Journal of Pharmaceutical Research, October 2010;
9(5):
499- 503
Abstract
Purpose:
To develop simple, rapid and selective
spectrophotometric methods for the determination of
cilostazol in tablet dosage form.
Methods:
Cilostazol was dissolved in 50% methanol and its
absorbance was scanned by ultraviolet (UV)
spectrophotometry. Both linear regression equation and
standard absorptivity were calculated and both methods
were validated as per ICH guidelines. Cilostazol was
determined in tablet dosage form using these validated
methods.
Results:
The l max of cilostazol was 258.2 nm in 50 % methanol. Beer-Lambert’s law was
obeyed in the concentration range of 0 – 25
mg/ml and standard absorptivity was 420.2 dL.g-1.cm-1.
The numerical values for all the validation
parameters were within acceptable limits. The results of
cilostazol tablet determination by linear regression
equation and standard absorptivity methods indicate
purity of 100.0 - 102.4 and 98.7 - 101.1 % with standard
deviations of 0.611 and 0.592, respectively. Comparing
the methods at 99 % confidence limit, the F-test value
was found to be 1.065.
Conclusion:
These
validated methods may be useful for routine analysis of
cilostazol as bulk drugs, in dosage forms as well as in
dissolution studies in the pharmaceutical industry.
Keywords:
Cilostazol tablets, UV spectrophotometry, Linear
regression equation, Standard absorptivity. |
