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Research
Article
Antioxidant and
Antihaemolytic Activities of the Leaves of Kefe cumin
(Laser trilobum L) Umbelliferae
Mohammad Ali Ebrahimzadeh1, Seyed Mohammad
Nabavi1,2, Seyed Fazel Nabavi1,
Bahman Eslami3, Zahra Rahmani4*
1Pharmaceutical
Sciences Research Center, School of Pharmacy,
Mazandaran University of Medical Sciences, Sari, 2Young
Researchers Club, Islamic Azad University, Ghaemshahr
Branch, Ghaemshahr, 3Department of Biology,
Islamic Azad University, Ghaemshahr branch, 4Faculty
of Medicine, Mazandaran University of Medical Sciences,
Sari, Iran.
*Corresponding author:
E-mail:
zadeh20@yahoo.com
Tel: +98-151-3543081-3; Fax: +98-151-3543084
Received: 9
January
2010
Revised accepted: 23 July 2010
Tropical
Journal of Pharmaceutical Research, October 2010;
9(5):
441- 449
Abstract
Purpose: To investigate the antioxidant
and antihaemolytic properties of the leaves of Laser
trilubum grown in Gaduk, Iran.
Methods:
The antioxidant and antihaemolytic activities of the
hydroalcohol extract of L. trilobum L. leaf were
investigated by haemoglobin-induced linoleic acid
peroxidation, scavenging of 1,1-diphenyl-2-picryl
hydrazyl (DPPH) radical, nitric oxide and hydrogen
peroxide, as well as by assessment of reducing power and
Fe2+ chelating activity, using standard
methods. Phenol and flavonoid contents were determined
as gallic acid and quercetin equivalents, respectively.
Results:
The extract showed antioxidant activity in some models,
with 50 % inhibitory concentration (IC50) for
DPPH radical-scavenging activity being 147.1 ± 7.5
mg
ml-1. The extract showed good nitric
oxide-scavenging activity of between 0.1 and 1.6 mg ml-1
(IC50 = 517.7 ± 23.1 vs. 20 ± 0.01 µg ml-1
for quercetin), weak Fe2+ chelating ability
(IC50, 906.9 ± 37.8
mg
ml-1), and low antioxidant activity in
haemoglobin-induced linoleic acid system. However, it
was capable of scavenging hydrogen peroxide in a
concentration-dependent manner while also exhibiting
potent antihaemolytic activity against H2O2
- induced haemolysis (IC50, 169.6 ± 6.9
mg
ml-1). Total phenolic content was 75 ± 3 mg
gallic acid equivalent/g and total flavonoid content
59.2 ± 2.1 mg quercetin equivalent/g. The amounts
of gallic acid, quercetin and rutin were 0.99 ± 0.04,
0.63 ± 0.01 and < 0.10 μg/mg, respectively.
Conclusion:
L. trilobum exhibited good but varying levels of
antioxidant and antihaemolytic activities in nearly all
the models studied, when compared with controls.
Keywords:
Antioxidant activity, Laser trilobum, Antihaemolytic,
Flavonoids, Kefe cumin |
