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Research Article

 

In Vitro Antitumour Activity of Sesamum indicum Linn Flower Extracts

 

Hua Xu1, 2*, Yong-ping Wen1, Wen Zhao1 and Qing He1

1Pharmacy College of Jinan University, 2Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, Jinan University, Guangzhou, Guangdong, 510632, China

 

*Corresponding author:  E-mail: huax_mail@126.com  Tel: +86-020-38375176

 

Received: 17 March 2010                                                                   Revised accepted: 30 August 2010

Tropical Journal of Pharmaceutical Research, October 2010; 9(5): 455-462

 

Abstract

 

Purpose: To investigate the antitumour activity of Sesamum indicum L.

Methods All the fractions of the ethanol extract of Sesamum indicum L. were obtained by solvent refining technique. Methyl thiazolyl tetrazolium (MTT) method was used to test the cytotoxicity of the extracts on four different tumour cell lines - Hela, CNE-2, smmc-7721 and Kyse-410. The effect of Sesamum indicum L. on apoptosis and cell cycle of tumour cells was analyzed by flow cytometry.

Results The petroleum benzene fraction and diethyl ether fraction showed significant antitumour activity compared with the control (P<0.05). Typical hypodiploid apoptotic peak was demonstrated in tumour cells treated by petroleum benzene fraction. The diethyl ether fraction evidently influenced the cell cycle of the tumour cells.

Conclusion The petroleum benzene and diethyl ether fractions of Sesamum indicum L. may contain antitumour components. The mechanism of inhibition of tumour cells may be linked to cytotoxic activity and apoptotic induction.

 

Keywords Sesamum indicum L.; Anti-tumour activity; MTT method; Flow cytometry.

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