http://dx.doi.org/10.4314/tjpr.v11i6.15
Abstract
Purpose: To determine whether L. pentosus
KCA1 can be used to create a new probiotic yogurt and
the organism’s duration of survival when stored at 4
oC.
Methods: Mother cultures of Lactobacillus
delbrueckii subsp. bulgaricus and Streptococcus
thermophilus and L. pentosus KCA1 were prepared and
subsequently added to a suspension of pasteurized milk.
It was then incubated at 37 oC for 6 h, after
which it was removed and placed in a refrigerator at 4
oC. Survival test was determined in MRS agar
plate supplemented with 30 µg of tetracycline for the
selective enumeration of L. pentosus KCA1 at
predetermined intervals over a period of 63 days at 4
oC.
Results: pH decreased both in normal yogurt
and probiotic yogurt and there was no significant
difference (p > 0.05) in the pH of the two preparations.
The strain showed higher viability for 49 days,
indicating the presence of a sufficient number of viable
bacterial cells at 4 oC. There were only 3
log cycle losses in the number of cells surviving from
day 1 (5.6 x 109 cfu/ml) to day 49 (5.5 x 106
cfu/ml).
Conclusion: This study shows that yogurt has
the potential to deliver biotherapeutic benefits
associated with probiotic bacteria to consumers
adequately.
Keywords:
Probiotics, Lactobacillus pentosus KCA1, Yogurt, Health
benefit