Improved
Production of Pharmacologically-active Sclerotiorin by
Penicillium sclerotiorum
Esther MF Lucas1,2,
Yuri Machado1, Alexandre A Ferreira1,
Luiza MP Dolabella1 and Jacqueline A
Takahashi1*
1Departamento
de Química, ICEx, Universidade Federal de Minas Gerais,
Av. Antonio Carlos, 6627, CEP 31270-901, Belo Horizonte,
MG, 2Departamento de Química, Universidade
Federal de Lavras, Campus Universitário, Centro, CEP
37200-000, Lavras MG, Brazil
*Corresponding author:
E-mail:
jat@qui.ufmg.br
Tel: +5531 34095754; Fax: +5531 34095700
Received: 1 October
2009
Revised accepted: 8 May 2010
Tropical
Journal of Pharmaceutical Research, August 2010;
9(4):
365-
371
Abstract
Purpose:
The aim of this work was to study the optimum conditions
for the production of sclerotiorin, a highly active
secondary metabolite of Penicillium sclerotiorum under
different cultural media.
Methods:
A Brazilian strain of P. sclerotiorum was grown under
different culture conditions in two liquid media (malt
and a dextrose-peptone salt medium supplemented with
sodium chloride) and in solid state fermentation in
rice. Sclerotiorin production was monitored by
high performance liquid
chromatography (HPLC).
Results:
Quantitative analysis of
sclerotiorin content by HPLC indicated that sclerotiorin
production reached the highest level (up to 313 +
10 mg.L-1) in the dextrose-based medium after
10 days of fermentation. Rice and malt broth showed
lower production levels.
Conclusion:
Enhanced production of P. sclerotiorin for
pharmaceutical development can be achieved using
dextrose-based cultures.
Keywords:
Penicillium sclerotiorum, Sclerotiorin,Yield
improvement, HPLC, Pharmaceutical industry
