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Research
Article
Production of
Antibiotics from Soil-Isolated Actinomycetes and
Evaluation of their Antimicrobial Activities
Basavaraj K Nanjwade1*,
S Chandrashekhara2, Prakash S Goudanavar2,
Ali M Shamarez1
and Fakirappa
V Manvi1
1KLES�s
College of Pharmaceutical Sciences, K.L.E. University,
Nehru Nagar, Belgaum,
2KLES�s
College of Pharmaceutical Sciences, Nipani,
Karnataka, India.
*Corresponding author:
E-mail:
bknanjwade@yahoo.co.in Tel:
+91-8312471399; Fax: +91-8312472387
Received: 30 November
2009
Revised accepted: 14 July 2010
Tropical
Journal of Pharmaceutical Research, August 2010;
9(4):
373-377
Abstract
Purpose:
To investigate the production of antibiotic from
actinomycetes isolated from soil and evaluate its
antimicrobial activities.
Methods:
In a medium formulation study, A-4 and A-4 actinomycete
mutant strains (out of the six strains selected from the
nine actinomycetes that were screened) were evaluated
for maximum antibiotic production using various carbon
and nitrogen sources. Zone of inhibition and packed cell
volume were the parameters used for the evaluation.
Various fermentation conditions such as pH, temperature
and dissolved oxygen were also optimized for maximal
production of antibiotic from both A-4 and A-4 mutant.
Results:
Some actinomycetes strains showed promising
antimicrobial activity against different strains of
bacteria and fungi. Out of the six strains selected, one
strain, designated A-4, showed maximum antimicrobial
property against Gram positive and Gram negative strains
as well as against various fungi.
Conclusion:
Findings from this investigation reveal that strain A-4
and A-4 mutant strains, in that order, exhibited
superior antimicrobial activities to other soil isolates
of actinomycetes.
Keywords:
Actinomycete,
Antibiotic, MIC, fermentation. |